Novel insights into the evolution of the caveolin superfamily and mechanisms of antiapoptotic effects and cell proliferation in lamprey.

摘要: Abstract Caveolin-1 is the main structural and functional component of caveolin, it involved in regulation cholesterol transport, endocytosis, signal transduction. Moreover, changes caveolin-1 play an important role tumorigenesis inflammatory processes. Previous studies have demonstrated that human mainly located cell membrane exhibits type- stage-dependent differences during cancer development responses. However, Lamprey-caveolin-like (L-caveolin-like) lamprey remained unknown. Here, we L-caveolin-like performs anti-inflammation oncogenic functions function diverged vertebrate evolution. results reveal mechanism underlying antiapoptotic effects L-caveolin-like. An gene from Lampetra japonica (L. japonica) was identified characterized. L-Caveolin-like primarily distributed leukocytes, intestines supraneural bodies (Sp-bodies) immune organs as indicated by Q-PCR immunohistochemistry assays. The mRNA protein expression levels L-caveolin exhibited consistent increases at 2 72 h adult tissues after exposure to lipopolysaccharide (LPS) leukocytes stimulated Vibrio anguillarum (V. anguillarum), Staphylococcus aureus (S. aureus), Poly I:C. Furthermore, overexpression pEGFP-N1-L-caveolin-like associated with a distinct localization mitochondria, decreased cytochrome C (Cyt C) mitochondrial Cyt oxidase subunit I (CO I) expression. In addition, increased cellular ATP suggested this prevented damage. led altered factors related apoptosis, such Caspase-9, Caspase-3, p53, Bax Bcl-2 promoted proliferation upregulated EGF, bFGF, PDGFB Together, these findings L. induced activation via C-mediated Caspase-3 signaling pathway. Our analysis further suggests oncogene product anti-inflammatory molecule evolved early