Porous glass as a solid support for immobilisation or affinity chromatography of enzymes.
作者： P.J. Robinson , P. Dunnill , M.D. Lilly
DOI: 10.1016/0005-2744(71)90160-4
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摘要: Abstract Chymotrypsin (EC 3.4.4.5) and β-galactosidase (gb-D-galactoside galactohydrolase, EC 3.2.1.23) were immobilised by attachment with glutaraldehyde to aminoalkylsilyl glass. The preparations contained 16 12 mg protein/g glass respectively. retention of activity on immobilisation for chymotrypsin was 50% 36%. Glycyl-D-phenylalanine in the same way used purification carboxypeptidase A (peptidyl-L-amino-acid hydrolase, 3.4.1.2) affinity chromatography.
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