Novel cardioprotective strategy combining three different preconditioning methods to prevent ischemia/reperfusion injury in aged hearts in an improved rabbit model

摘要: The use of ischemic preconditioning (IPC) to protect the myocardium is usually not effective in elderly patients. aim present study was design new methods achieve enhanced myocardial protection, based on differential role endogenous adenosine (ADO) and ADO receptors (ARs) effects IPC young old animals. An improved New Zealand white rabbit model ischemia/reperfusion established Langendorff model. Adult or hearts, with without exposure IPC, were used order assess roles ARs different IPC. Different protective designed a combination exogenous interventions. Cardiac function, as well biochemical, histopathological apoptotic indices, measured intervention groups. stable, reliable suitable for undertaking experiments. levels aged hearts pre- post-IPC lower than those adult indicating that may be an factor influencing A protection strategy combining ADO-enhanced A1AR agonist 2-chloro-N(6)-cyclopentyladenosine cold crystalloid cardioplegia had significant effect hearts. results suggested enhancement, treatment yield additive simultaneous application these three types provided most heart