Bone loss prevention in ovariectomized rats using stable amorphous calcium carbonate

摘要: In assessing the relationship between calcium supplementation and maintaining bone mass or reducing risk of fracture, effectiveness of has never been decisive. Freshwater crayfish rely on amorphous calcium carbonate (ACC), an instable polymorph carbonate, as main mineral in exoskeleton in temporary storage organ, gastrolith. Inspired by model, we have previously shown increase bioavailability in rats administered with synthetic stable ACC vs. crystalline carbonate (CCC). The current study compared effects derived from either gastrolith those calcium, found in commercial CCC calciumcitrate supplements, in a loss prevention model. Rats were subjected to either sham ovariectomy (OVX) operation (n~20/ group) followed by administration food pellets supplemented with 0.5% from source over 12 weeks. Micro-computed tomography (μCT) histomorphometric analyses revealed both treatments, manifested morphometric parameters, CCC- and citrate-treated groups. Both treatments resulted formation tibia cancellous bone, indicated dynamic histomorphometry citrate treatments. Levels urine deoxypyridinoline (DPD), suggested an anti-resorptive effect ACC, which was also only treatment that led significant vertebral mechanical strength, supported by μCT analysis topology orientation parameters trabeculae. To our knowledge, such levels bone loss supplements never been reported. These findings thus suggest potential natural (crayfish gastrolith) and, greater extent, sources for prevention of metabolic disorders possibly osteoporotic processes.