Functional and molecular analysis of hematopoietic progenitors derived from the aorta-gonad-mesonephros region of the mouse embryo.

摘要: Herein, we show that CD34, c-kit double-positive (CD34+c-kit+) cells from the aorta-gonad-mesonephros (AGM) region of developing mouse are multipotent in vitro and can undergo both B-lymphoid multimyeloid differentiation. Molecular analysis individual CD34+c-kit+ by single-cell reverse transcriptase–polymerase chain reaction (RT-PCR) shows coactivation erythroid (β-globin) myeloid (myeloperoxidase [MPO]) but not lymphoid-affiliated (CD3, Thy-1, λ5) genes. Additionally, most coexpress stem cell–associated transcriptional regulators AML-1, PU.1, GATA-2 Lmo2, as well granulocyte colony-stimulating factor receptor (G-CSF-R). These results population AGM represents a highly enriched source hematopoietic cells, suggest limited distinct lineage-affiliated genes is an early event generation progenitor during ontogeny.