Primary structure of c-kit: relationship with the CSF-1/PDGF receptor kinase family-oncogenic activation of v-kit involves deletion of extracellular domain and C terminus

摘要: The protein kinase domains of v-kit, the oncogene acute transforming feline retrovirus HZ4-FeSV (HZ4-feline sarcoma virus), CSF-1R (macrophage colony stimulating factor receptor) and PDGFR (platelet derived growth display extensive homology. Because close structural relationship we predicted that c-kit would encode a transmembrane receptor (Besmer et al., 1986a; Yarden 1986). We have now determined primary structure murine from DNA clone isolated brain cDNA library. nucleotide sequence predicts 975 amino acid product with calculated mol. wt 109.001 kd. It contains an N-terminal signal peptide, domain (residues 519-543) in C-terminal half v-kit homologous sequences 558-925). therefore features which are characteristic kinase. Comparison c-kit, revealed unique these kinases suggesting common evolutionary origin. outer cellular was shown to be related immunoglobulin superfamily. sites expression normal tissue predict function hematopoietic cells. include extracellular as well 50 acids C-terminus deleted v-kit. These alterations likely determinants oncogenic activation v-kit.(ABSTRACT TRUNCATED AT 250 WORDS)