Nucleic acid sequence and oncogenic properties of the HZ2 feline sarcoma virus v-abl insert.

摘要: Hardy-Zuckerman 2 feline sarcoma virus (HZ2-FeSV), isolated from a multicentric fibrosarcoma is replication-defective acute transforming retrovirus which originated by transduction of c-abl sequences with leukemia (FeLV) and known to encode 110-kilodalton gag-abl fusion protein tyrosine-specific kinase activity (P. Besmer, W. D. Hardy, E. Zuckerman, P. J. Bergold, L. Lederman, H. Snyder, Nature (London) 303:825-828, 1983). The nucleotide sequence the abl segment in HZ2-FeSV genome was determined compared murine human v-abl sequences. predicted consists 344 amino acids (aa) FeLV gag origin, 439 aa at least 200 pol origin (p110gag-abl-pol). 1,317-base-pair (fv-abl) corresponds 59 include region specify domain. 189 base pairs fv-abl correspond not contained Abelson (MuLV) v-abl. mouse exon contains these segments identified, its determined. Comparison acid those MuLV revealed five differences. junction between found involve preferred p30 Murphy, C. George, F. Qiu, Brodeur, 320:415-421, 1986). A six-base homology exists recombination site parental 39 predicts an in-frame pol. transformed cell line containing truncated gag-abl-pol protein, p85, that lacks most obtained transfection NIH 3T3 cells. This result implies p110gag-abl-pol are dispensable for fibroblast transformation. To assess whether specifies unique biological properties HZ2-FeSV, we constructed Moloney MuLV-based version Mo-MuLV(fv-abl), were genetic context similar HZ2-FeSV.(ABSTRACT TRUNCATED AT 400 WORDS) Images