Saliva: a convenient source of DNA for analysis of bi-allelic polymorphisms of Fcγ receptor IIA (CD32) and Fcγ receptor IIIB (CD16)

摘要: Abstract Genetic polymorphisms of low-affinity IgG Fc receptors (Fc γ R) have been found to influence binding human subclass antibodies, and may susceptibility certain types infectious autoimmune diseases. Phenotypic and/or genotypic analyses R traditionally employed peripheral venous blood as a source leukocytes or genomic DNA, respectively. The present study was undertaken determine whether salivary DNA is suitable alternative extracted from for genetic analysis allelic polymorphisms. Genomic whole saliva 69 healthy adult volunteers using commercial purification kit. average quantity isolated per ml 19.2±14.1 μ g. To assess intrasubject variation in yield ten samples were collected single donor over 3-month period. recovered these 25.2±13.7 Volumes small 100 l, well stored at −70°C prolonged periods (up 6 years), provided amounts sufficient PCR-based analysis. Two comparative PCR assays performed both number individuals. able detect nucleotide substitution (G→A) the RIIA gene, two codominant alleles encoding NA polymorphism RIIIB, Furthermore, RIIIB genotype results confirmed by quantitative flow cytometry specific monoclonal antibodies. Complete concordance achieved between typing our DNA-based assays. Therefore, appears be an excellent studies