Rapid extraction of genomic DNA from saliva for HLA typing on microarray based on magnetic nanobeads
作者： Xin Xie , Xu Zhang , Bingbin Yu , Huafang Gao , Huan Zhang
DOI: 10.1016/J.JMMM.2004.03.008
关键词: DNA 、 Oligonucleotide 、 Saliva 、 Computational biology 、 Chemistry 、 genomic DNA 、 Human leukocyte antigen 、 DNA extraction 、 Microarray 、 Extraction (chemistry)
摘要: Abstract A series of simplified protocols are developed for extracting genomic DNA from saliva by using the magnetic nanobeads as absorbents. In these protocols, both enrichment target cells and adsorption can be achieved simultaneously our functionally modified beads in one step, DNA–nanobeads complex used PCR templates. HLA typing based on an oligonucleotide array was conducted hybridization with products. The result shows that robust sensitive.
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