Polyelectrolyte functionalized magnetic emulsion for specific isolation of nucleic acids.
作者： Raphael Veyret , Abdelhamid Elaissari , Thierry Delair
DOI: 10.1016/J.COLSURFB.2006.08.004
关键词:
摘要: A magnetic oil in water (o/w) emulsion was cationized by adsorption of poly(ethyleneimine) (PEI). In a subsequent step, the cationic particles were derivatized with partially hydrolyzed poly(maleic anhydride-alt-methyl vinyl ether) copolymer (PMAMVE) to lead negatively charged colloids. The experimental conditions for covalent grafting PMAMVE selected on basis colloidal stability, charge inversion and absence inhibition enzymatic DNA/RNA amplification reactions. Once selected, oligonucleotides (ODN) bearing obtained according sequential process: (i) single stranded ODNs onto PMAMVE; (ii) PMAMVE-ODN conjugates defined above. this strategy, both steps could be independently controlled. ODN-PMAMVE-particles very stable time, did not inhibit RT-PCR capable hybridizing specifically complementary target.
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