Magnetic Nanoparticles PCR Enzyme‐Linked Gene Assay for Quantitative Detection of BCR/ABL Fusion Gene in Chronic Myelogenous Leukemia

摘要: Background Magnetic nanoparticles (MNPs) have been widely used in medical diagnostic research. In this work, two technologies, MNPs and polymerase chain reaction (PCR), were combined to increase detection sensitivity specificity. A novel technique based on the MNPs-PCR enzyme-linked gene assay (MELGA) was developed for of BCR/ABL abnormal chronic myelogenous leukemia (CML) patients. Methods An MNPs-labeled BCR forward primer a biotin-labeled ABL reverse specifically amplify target gene. After magnetic separation, PCR product bound labeled with streptavidin-conjugated horseradish peroxidase incubated substrate hydrogen peroxide generate colorimetric signal. Results When compared real-time quantitative-PCR (RQ-PCR), MELGA exhibited an increased <1 fg high specificity fusion CML patients. addition, results correlated well number copies obtained from RQ-PCR. Conclusion This simple cost-effective is suitable monitoring patients during targeted therapy (tyrosine kinase inhibitors) especially rural hospitals.