Combination of PCR and dual nanoparticles for detection of Plasmodium falciparum.

摘要: Highly reactive particle-based DNA amplification was developed for the detection of Pfg377 gene from P. falciparum gametocytes using functional magnetic latex particles (MLPs) and quantum dots encapsulated polymer (QDs-PPs). Firstly, MLPs were prepared precipitation iron oxide, polymerization initiator, adsorption aminodextran (AMD) so as to provide amino-functionalized MLPs. Furthermore, amino-containing (PPs) by emulsifier-free with fluorescent (QDs) use a signaling support. Subsequently, poly(maleic anhydride-alt-methyl vinyl ether) (PMAMVE) copolymer effectively used rapid simple grafting amino-modified primers onto surface thereby providing promising method particle immobilization. Herein, primer-grafted applied in PCR approach. After amplification, products containing PMAMVE-grafted QDs-PPs separated magnet examined via fluorescence microscope. not found inhibit reaction while facilitating efficient product. Results showed high sensitivity specificity amplified within only few steps. This procedure represents novel improvement post-amplification analysis.