Wheat germ agglutinin stains dispersed post-golgi vesicles after treatment with the cytokinesis inhibitor psychosine.
作者： Takayuki Kanazawa , Hiromu Takematsu , Akitsugu Yamamoto , Harumi Yamamoto , Yasunori Kozutsumi
DOI: 10.1002/JCP.21328
关键词:
摘要: The galactosylsphingosine psychosine (Psy) is one of the sphingolipids and induce formation multinuclear cells in several cell lines by inhibiting cytokinesis. In present report, we show that intracellular organelles, including wheat germ agglutinin (WGA)-positive vesicles early endosomes, are selectively dispersed Psy. WGA a conventional Golgi marker WGA-positive appeared to co-localize with apparatus untreated cells. Psy treatment induced dispersal without affecting structure apparatus, resulting discrimination from apparatus. sharp contrast this effect Psy, were not affected brefeldin A treatment, which disappearance Immunostaining anti-TGN46 antibodies revealed large portion derived trans-Golgi network. Notably, did stain anti-syntaxin 6, another During cytokinesis, cytoplasm decreased, staining accumulated at cleavage furrow, was apparently inhibited presence These data suggest transport furrow associated progression
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