Loss of Free Amino Groups in the Water-insoluble Fraction of Nuclear Senile Cataracts
作者： M N Miranda-Rivera , S Garćia-Castiñeiras
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摘要: Free amino groups were analyzed in the water-insoluble fraction (WIF) of cataractous human lenses nuclear sclerosis, pigmented type. Two modified versions trinitrobenzene sulfonic acid (TNBS) procedure for determination free proteins (AFSA Habeeb, Anal Biochem 14:328-336, 1966) used this purpose. The concentration WIF was found to be inversely related dry weight fraction. Taking as a measure severity cataract, it can thus said that relative loss apparently occurs during insolubilization process associated type senile cataract. disappearance rate has been estimated about 0.9% — NH2 per 1 mg material added WIF. After considering other possible alternatives (loss accessibility TNBS, dilution by materials with low primary amine content) we have interpreted finding most likely due postsynthetic blockade lysine e-amino functions proteins. potential involvement residues Schiff base formation is discussed within context current views cataractogenesis. reaction e-NH2 unidentified carbonyl compounds could represent partial but quantitatively important mechanism yellowing cataracts. Invest Ophthalmol Vis Sci 24:1181-1187, 1983 Postsynthetic modifications are amply represented lens peculiarities its metabolism and growth. following described: racemization * 2 ; deamidation 34 oxidation sulphur 5 " 7 also probably aromatic aminoacids 8 glycosylation 9 polypeptide chain cleavage 310 disulfide nondisulfide cross-linking"" 13 appearance visible fluorescence. 14 18 We reporting new kind post-synthetic modification proteins, namely, which detected waterinsoluble Materials Methods
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