The reaction of formaldehyde with nucleotides and T2 bacteriophage DNA

摘要: The mechanism of ribo- and deoxyribonucleotide reaction with formaldehyde has been examined respect to kinetics, pH optima structural specificity. Formaldehyde reacts only With those nucleotides bearing amino groups. equilibrium constants for the as well rate forward reverse directions have described. difference between reactivity deoxyribo-nucleotides toward is negligible exception guanine deoxyribo-nucleotides. That 5′-dGMP at a faster than 5′-GMP attributed steric interference group hydroxymethylation protonation by 2′-hydroxyl group. Moreover nucleotide appears be pH-dependent since base-catalysed. effect on DNA thermal transitions was studied using spectrophotometric, viscometric immunological methods. Hydroxymethylation preceded denaturation lowers temperature requirements transition. depression requirement bacteriophage native denatured nitrogens associated purine pyrimidine rings.