Phylogenetic diversity of a microbialite reef in a cold alkaline freshwater lake

摘要: A culture-independent multidomain survey of biodiversity in microbialite structures within the cold alkaline Pavilion Lake (British Columbia, Canada) revealed a largely homogenous community at depths from 10 to 30 m. Real-time quantitative PCR was used demonstrate that bacteria comprised approximately 80%–95% recoverable phylotypes. Archaeal phylotypes accounted for <5% microbialites exposed water column, while sediment contact supported 4- 5-fold higher archaeal abundance. Eukaryal were rare and indicated common aquatic diatoms concluded not be part community. Phylogenetic analysis rRNA genes clone libraries (N = 491) alphaproteobacterial most abundant. Cyanobacterial highly diverse but resolved into 4 dominant genera: Acaryochloris, Leptolyngbya, Microcoleus, Pseudanabaena. Interestingly, cyanobacteria generally affiliated phylogenetically w...