Determinação do perfil fitoquímico de extrato com atividade antioxidante da espécie medicinal Cordia verbenacea DC. por HPLC-DAD
作者： M.M. Santi , F.S. Sanches , J.F.M. Silva , P.M.L. Santos
DOI: 10.1590/S1516-05722014000200014
关键词:
摘要: The objective of the present study was to investigate antioxidant activity extracts from Cordia verbenacea leaves obtained by maceration in ethanol and partitioned with organic solvents. infusion also investigated. total phenolic flavonoid content evaluated Folin-Ciocalteau method aluminum chloride complex method, respectively. extract, subfractions were assayed at various concentrations determine DPPH scavenging expressed terms CE50. best found on ethyl acetate EA, CE50 15.0 ± 0.5 µg.mL-1. spectrophotometric assays revealed high phenol EA extract. HPLC-DAD analysis performed reveal UV-Vis profile chromatographic peaks UV spectral characteristics related polyphenol compounds.
academicoo.com参考文章(18)
Marcela AS Coutinho, Michele F Muzitano, Sônia S Costa, None, Flavonoides: Potenciais agentes Terapêuticos para o Processo Inflamatório Revista Virtual de Química. ,vol. 1, pp. 241- 256 ,(2009) , 10.5935/RVQ.V1I3.51
Ricardo G Woisky, Antonio Salatino, Analysis of propolis: some parameters and procedures for chemical quality control Journal of Apicultural Research. ,vol. 37, pp. 99- 105 ,(1998) , 10.1080/00218839.1998.11100961
Luciana L Mensor, Fábio S Menezes, Gilda G Leitão, Alexandre S Reis, Tereza C dos Santos, Cintia S Coube, Suzana G Leitão, None, Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method Phytotherapy Research. ,vol. 15, pp. 127- 130 ,(2001) , 10.1002/PTR.687
Mahinda Wettasinghe, Fereidoon Shahidi, Ryszard Amarowicz, Mamdouh M. Abou-Zaid, Phenolic acids in defatted seeds of borage (Borago officinalis L.) Food Chemistry. ,vol. 75, pp. 49- 56 ,(2001) , 10.1016/S0308-8146(01)00182-0
Erika de Freitas Roldão, Aline Witaicenis, Leonardo Noboru Seito, Clélia Akiko Hiruma-Lima, Luiz Claudio Di Stasi, Evaluation of the antiulcerogenic and analgesic activities of Cordia verbenacea DC. (Boraginaceae) Journal of Ethnopharmacology. ,vol. 119, pp. 94- 98 ,(2008) , 10.1016/J.JEP.2008.06.001
Fábio K Ticli, Lorane IS Hage, Rafael S Cambraia, Paulo S Pereira, Ângelo J Magro, Marcos RM Fontes, Rodrigo G Stábeli, José R Giglio, Suzelei C França, Andreimar M Soares, Suely V Sampaio, None, Rosmarinic acid, a new snake venom phospholipase A2 inhibitor from Cordia verbenacea (Boraginaceae): antiserum action potentiation and molecular interaction Toxicon. ,vol. 46, pp. 318- 327 ,(2005) , 10.1016/J.TOXICON.2005.04.023
Sergio Eduardo SOARES, Ácidos fenólicos como antioxidantes Revista De Nutricao-brazilian Journal of Nutrition. ,vol. 15, pp. 71- 81 ,(2002) , 10.1590/S1415-52732002000100008
L. E. Dowd, Spectrophotometric Determination of Quercetin Analytical Chemistry. ,vol. 31, pp. 1184- 1187 ,(1959) , 10.1021/AC60151A033
F. Geller, C. Schmidt, M. Göttert, M. Fronza, V. Schattel, B. Heinzmann, O. Werz, E.M.M. Flores, I. Merfort, S. Laufer, Identification of rosmarinic acid as the major active constituent in Cordia americana Journal of Ethnopharmacology. ,vol. 128, pp. 561- 566 ,(2010) , 10.1016/J.JEP.2010.01.062
Eliane M.Z. Michielin, Luiz Paulo de Lemos Wiese, Eduardo Antonio Ferreira, Rozangela Curi Pedrosa, Sandra R.S. Ferreira, Radical-scavenging activity of extracts from Cordia verbenacea DC obtained by different methods Journal of Supercritical Fluids. ,vol. 56, pp. 89- 96 ,(2011) , 10.1016/J.SUPFLU.2010.11.006