Separation of Cell Types from the Cerebellum and Their Properties

摘要: Publisher Summary This chapter discusses the disassociation of cell types from cerebellum and their individual properties. The constituent functional units a brain can be separated in pure viable form, thereby enabling properties to studied isolation.The dissociation procedure is short duration (1hr) carried out using media that are isotonic, well-oxygenated, at physiological pH; lengthy centrifugation steps high-gravitational forces avoided, traumatic process mechanical dispersion facilitated by pretreatment tissue with trypsin.The samples sliced incubated or without enzymes prior dissociation; ability obtain cells slices depends, first instance, on being viable. These readily grow differentiate culture, thus, allowing investigation into, for example, mechanisms underlying developmental events such as synaptogenesis. In an experiment described chapter, competence isolated was evidenced sustained glycolysis coupled respiration, maintenance normal transmembrane gradients Na + K , culture. Another method obtaining purified populations use culture techniques; choice conditions selected preferential survival particular types, cultures greatly enriched either interneurons astrocytes were obtained earlier study.