Expression of BCL-2 Protein Enhances the Survival of Mouse Fibrosarcoid Cells in Tumor Necrosis Factor-mediated Cytotoxicity

摘要: Abstract Tumor necrosis factor (TNF) kills some types of tumor cells in vitro and participates elimination vivo . TNF has been shown to kill by altering their mitochondria structurally functionally. The oncogene BCL-2 codes for a protein located the inner membrane which is able inhibit commitment cell death various types. We have therefore investigated whether TNF-mediated killing line L929 could be modulated expression BCL-2. report here that transfected with vector an increased survival compared wild type after challenge. protective effect greatest at moderate concentrations still significant killed 100% cells. action selective inasmuch as are not protected against other cytotoxic agents blocking mitochondrial functions. show expressing higher potential (ΔΨ) than increase ΔΨ linked enhanced Indeed, we found treatment ionophore nigericin, increases ΔΨ, protects them even high concentrations.