A sheep model for the study of biofilms in rhinosinusitis.

摘要: BackgroundBacterial biofilms have been shown in chronic diseases such as cystic fibrosis, cholesteatoma, and otitis media with effusion. Recently, their detection on the mucosal tissue of sinusitis patients has implicated them pathogenesis this condition. We present an animal model using sheep experimentally infected Staphylococcus aureus to study possible association between biofilm sinusitis.MethodsTwenty-four underwent bilateral endoscopic sinus surgery identify frontal ostia. The sinuses were treated one following ways according preoperative randomization: (1) ostium left patent, (2) patent bacteria instilled, (3) occluded, or (4) occluded instilled. mucosa was harvested at day 7 examined for presence confocal scanning laser microscopy (CSLM) well electron (SEM) transmission (TEM).ResultsAll three modalities showed differe...