Cell Viability Assays
作者： Sarah Duellman , Terry L Riss , Lisa Minor , Tracy J Worzella , Andrew L Niles
DOI:
关键词:
摘要: This chapter is an introductory overview of the most commonly used assay methods to estimate number viable cells in multi-well plates. describes assays where data are recorded using a plate-reader; it does not cover designed for flow cytometry or high content imaging. The covered include use different classes colorimetric tetrazolium reagents, resazurin reduction and protease substrates generating fluorescent signal, luminogenic ATP assay, novel real-time monitor live days culture. described based on measurement marker activity associated with cell number. These measuring results proliferation, testing cytotoxic effects compounds, multiplexing as internal control determine during other cell-based assays.
参考文章(26)
Michael V. Berridge, Patries M. Herst, An S. Tan, Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnology annual review. ,vol. 11, pp. 127- 152 ,(2005) , 10.1016/S1387-2656(05)11004-7
Abby C. Collier, Chris A. Pritsos, The mitochondrial uncoupler dicumarol disrupts the MTT assay Biochemical Pharmacology. ,vol. 66, pp. 281- 287 ,(2003) , 10.1016/S0006-2952(03)00240-5
Lynn Huyck, Christophe Ampe, Marleen Van Troys, The XTT Cell Proliferation Assay Applied to Cell Layers Embedded in Three-Dimensional Matrix Assay and Drug Development Technologies. ,vol. 10, pp. 382- 392 ,(2012) , 10.1089/ADT.2011.391
M.V. Berridge, A.S. Tan, Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT): subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction Archives of Biochemistry and Biophysics. ,vol. 303, pp. 474- 482 ,(1993) , 10.1006/ABBI.1993.1311
M.C. Pagliacci, F. Spinozzi, G. Migliorati, G. Fumi, M. Smacchia, F. Grignani, C. Riccardi, I. Nicoletti, Genistein inhibits tumour cell growth in vitro but enhances mitochondrial reduction of tetrazolium salts: A further pitfall in the use of the MTT assay for evaluating cell growth and survival European Journal of Cancer. ,vol. 29, pp. 1573- 1577 ,(1993) , 10.1016/0959-8049(93)90297-S
Andrew L. Niles, Richard A. Moravec, P. Eric Hesselberth, Michael A. Scurria, William J. Daily, Terry L. Riss, A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers Analytical Biochemistry. ,vol. 366, pp. 197- 206 ,(2007) , 10.1016/J.AB.2007.04.007
Lanhai Lü, Lihong Zhang, Maria Sen Mun Wai, David Tai Wai Yew, Jie Xu, Exocytosis of MTT formazan could exacerbate cell injury Toxicology in Vitro. ,vol. 26, pp. 636- 644 ,(2012) , 10.1016/J.TIV.2012.02.006
David Shum, Constantin Radu, Earl Kim, Muriel Cajuste, Yufang Shao, Venkatraman E. Seshan, Hakim Djaballah, A high density assay format for the detection of novel cytotoxic agents in large chemical libraries Journal of Enzyme Inhibition and Medicinal Chemistry. ,vol. 23, pp. 931- 945 ,(2008) , 10.1080/14756360701810082
Engin Ulukaya, Mukaddes Colakogullari, Edward J. Wood, Interference by Anti-Cancer Chemotherapeutic Agents in the MTT-Tumor Chemosensitivity Assay Chemotherapy. ,vol. 50, pp. 43- 50 ,(2004) , 10.1159/000077285
Hiroko Tada, Osamu Shiho, Ken-ichi Kuroshima, Masaru Koyama, Kyozo Tsukamoto, An improved colorimetric assay for interleukin 2. Journal of Immunological Methods. ,vol. 93, pp. 157- 165 ,(1986) , 10.1016/0022-1759(86)90183-3