Interaction of Intestinal Bacteria with Human Rotavirus during Infection in Children.

摘要: The gut microbiota has emerged as a key factor in the pathogenesis of intestinal viruses, including enteroviruses, noroviruses and rotaviruses (RVs), where stimulatory inhibitory effects on infectivity have been reported. With aim determining whether members interact with RVs during infection, combination anti-RV antibody labeling, fluorescence-activated cell sorting 16S rRNA amplicon sequencing was used to characterize interaction between specific bacteria RV stool samples children suffering from diarrhea produced by G1P[8] RV. genera Ruminococcus Oxalobacter were identified binders stools, displaying enrichments 4.8- 5.4-fold compared nonlabeled antibodies. In vitro binding Wa human strain two gauvreauii isolates confirmed fluorescence microscopy. Analysis R. antibodies directed several histo-blood group antigens (HBGAs) indicated that these express HBGA-like substances their surfaces, which can be target for binding. Furthermore, infection differentiated Caco-2 cells significantly reduced incubation gauvreauii. These data, together previous findings showing negative correlation levels titers healthy individuals, suggest pivotal this bacterial results reveal likely mechanisms how taxa could negatively affect open new possibilities antiviral strategies.