The isolation of two mutants of Saccharomycescerevisiae which demonstrate increased activity of 3-hydroxy-3-methylglutaryl coenzyme a reductase
作者： James F. Downing , Linda S. Burrows , Martin Bard
DOI: 10.1016/0006-291X(80)91330-3
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摘要: Abstract Two mutants of Saccharomyces cerevisiae have been isolated which demonstrate increased levels 3-hydroxy-3-methylglutaryl coenzyme A reductase activity. The activity this enzyme was paralleled by increases in sterol production. were selected virtue their resistance to the cytotoxic effects 6-ketocholestanol. It shown that results from decreased permeability cells oxygenated analog. Gas-liquid chromatographic analyses sterols present these strains implicate conversion squalene into as an important regulatory step biosynthesis yeast.
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