Novel G3/DT adjuvant promotes the induction of protective T cells responses after vaccination with a seasonal trivalent inactivated split-virion influenza vaccine

摘要: Vaccines used against seasonal influenza are poorly effective A viruses of novel subtypes that may have pandemic potential. Furthermore, pre(pandemic) vaccines immunogenic, which can be overcome by the use adjuvants. limited number adjuvants has been approved for in humans, however there is a need alternative safe and enhance immunogenicity promote induction broad-protective T cell responses. Here we evaluated nanoparticle, G3, as an adjuvant trivalent inactivated vaccine mouse model. The G3 was formulated with or without steviol glycosides (DT, diterpenoid). both formulations enhanced virus-specific antibody response to all three strains considerably. were well tolerated any signs discomfort. To assess protective potential vaccine-induced immune responses, antigenically distinct virus strain, A/Puerto Rico/8/34 (A/PR/8/34), challenge infection. antibodies did not cross-react strain A/PR/8/34 HI VN assays. However, mice immunized G3/DT-adjuvanted partially protected infection, correlated anamnestic CD8(+) responses observed DT. Both induced maturation human dendritic cells promoted antigen presentation similar extent. In conclusion, G3/DT promising formulation only potentiates vaccines, but also induces immunity could afford broader protection viruses.