Genetic studies with tumorigenic adenoviruses: I. Isolation of cytocidal (cyt) mutants of adenovirus type 12

摘要: Abstract A class of cytocidal mutants, designated cyt have been isolated from two highly tumorigenic strains adenovirus type 12 (Ad. 12), i.e., Huie and 1131. Parental viruses were plated on cell plates low efficiency plating, such as human amnion FL embryonic kidney (HEK) diploid plates. Small clear plaques, distinct the majority minute parental isolated. After purification these plaques found to be mutants. cyt+ stocks contain approximately 0.001% spontaneous mutants recoverable by this method. Ultraviolet irradiation 10−1 10−2 survival increases proportion a factor about 5. In contrast adenovirus-type cytopathic effect (CPE) viruses, CPE produced is characterized cellular destruction. The produce large HEK plates, while form small fuzzy-edged plaques. Under conditions mixed infection dominant over CPE. are much less in newborn hamsters than fail transform hamster cells vitro. cooperate tumorigenicity with field well Ad. 7 or 3. Thus it may hypothesized that gene responsible both for production equivalent c1 temperate phage λ.