Arsenosugar identification in seaweed extracts using high-performance liquid chromatography/electrospray ion trap mass spectrometry

摘要: The development of analytical techniques suitable for providing structural information on a wide range elemental species is growing necessity. For arsenic speciation variety mass spectrometric techniques, mainly inductively coupled plasma spectrometry (ICP-MS) and electrospray tandem (ES-MS/MS) on-line with high-performance liquid chromatography (HPLC), are in use. In this paper we report the identification present samples marine origin (seaweed extracts) using ES ion trap (IT) multistage (MS(n)). Both reversed-phase anion-exchange HPLC have been to ES-ITMS. Product scans multiple stages MS (MS(n); n=2-4) were used acquire diagnostic data each arsenosugar. spectra contain structurally characteristic fragment ions arsenosugars examined. addition it was observed that upon successive collision-induced dissociation (CID) common product (m/z 237) formed from all four This has potential be as an indicator presence dimethylated (dimethylarsinoylribosides). HPLC/ES-ITMS(n) method developed allows sensitive crude seaweed extracts without need extended sample preparation. fact, preparation requirements identical those typically employed HPLC/ICP-MS analysis. Additionally, resulting examined, reproducible correspond well obtained other low-energy CID techniques. As result, approach minimises misidentification great means overcoming characterised standards.