Significant upregulation of U1 and U4 spliceosomal snRNAs by ATP nanoliposomes explains acceleration of wound healing, due to increased pre-mRNA processing to functional mRNA

摘要: Delayed wound healing is one of the hallmarks diabetic complications and certain autoimmune inflammatory diseases. Extensive studies in rabbits have indicated that delivery ATP encapsulated unilamellar nanoliposomes causes rapid cell proliferation fast tracks process. In current study, we explored possible molecular mechanism underlying this response by comparing gene expression cultured rabbit kidney cells treated with either (containing 1 mg Mg-ATP/ml formulation) or control mg/ml unmetabolisable gamma-thio-ATP/ml formulation). High-quality total RNA was isolated 24 h from subjected to seq technology, which revealed significant overexpression specific noncoding RNAs. The U1 spliceosomal RNA, snRNA, upregulated more than 250-fold following treatment nanoliposomes. This multifunctional may function transcription speeding up critical splicing step, thereby facilitating faster processing pre-mRNA translatable mRNA.