MicroRNAs Regulate Cellular ATP Levels by Targeting Mitochondrial Energy Metabolism Genes during C2C12 Myoblast Differentiation
作者： Puntita Siengdee , Nares Trakooljul , Eduard Murani , Manfred Schwerin , Klaus Wimmers
DOI: 10.1371/JOURNAL.PONE.0127850
关键词:
摘要: In our previous study, we identified an miRNA regulatory network involved in energy metabolism porcine muscle. To better understand the involvement of miRNAs cellular ATP production and metabolism, here used C2C12 myoblasts, which levels increase during differentiation, to identify modulating these processes. level, mRNA microarray expression profiles differentiation into myotubes were assessed. The results suggest 14 (miR-423-3p, miR-17, miR-130b, miR-301a/b, miR-345, miR-15a, miR-16a, miR-128, miR-615, miR-1968, miR-1a/b, miR-194) as regulators targeting genes mitochondrial (Cox4i2, Cox6a2, Ndufb7, Ndufs4, Ndufs5, Ndufv1) differentiation. Among these, miR-423-3p showed a high inverse correlation with increasing levels. Besides having implications promoting cell growth cycle progression, its function regulation is yet unknown. Therefore, was selected validated for together potential target, Cox6a2. Overexpression myogenic lead decreased level Cox6a2 compared negative control. These novel regulator ATP/energy by
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