Hydrolysis of whey protein isolate in a tangential flow filter membrane reactor: I. Characterisation of permeate flux and product recovery by multivariate data analysis

摘要: Abstract Protease N (IUB 3.4.24.28, Bacillus subtilis ) enzyme was used to continuously hydrolyse an initial 5% (w/v) whey protein isolate (86.98%, Kjeldahl nitrogen × 6.38) for 5 h at pH 7.0 and 55 °C in a 10 kDa tangential flow filter (TFF) enzymatic membrane reactor (EMR). The retentate temperature ( A : 25–55 °C), water permeate flux, J i B 1.6–18.4 mL/min) concentration C 0.5–5.5 g) were varied optimised using response surface methodology (RSM) central composite rotatable design (CCRD). residual activity ), leakage loss average flux nitrogen recovered (apparent sieving, S apparent determined. decayed extensively low temperatures (25 30 °C), while 50 °C the solubilised dynamic gel layer, stabilised led higher . increased concomitantly as well with increasing temperature, concentration. Principal components analysis isolated , factors providing prominent influence EMR significant contributions (ca. 60% of variance) principal 1 3 (permeate substrate hydrodynamics property). component 2 (‘measure’ property) contributed 27.78%. Results provide evidence that when feed is suitable, high solubility viscosity maintained solubilises hydrolyses polarisation layer (GPL) thus codetergence property necessary maintain stability hence product recovery.