Short communication: Detection of antibiotic resistance, mecA, and virulence genes in coagulase-negative Staphylococcus spp. from buffalo milk and the milking environment.

摘要: The aim of this study was to determinate whether coagulase-negative staphylococci (CNS) from buffalo milk or the milking environment possess virulence factors that are associated with intramammary infections antimicrobial resistance. Milk samples (n = 320) 80 lactating were evaluated for clinical and subclinical mastitis by physical examination, strip cup test, California Mastitis Test (CMT), somatic cell count (SCC) over a 4-mo period. In addition, swabs obtained hands consenting milkers (16), liners (64), mouths (15) nostrils calves. No cases observed; however, CMT together SCC results indicated 8 animals had mastitis. Eighty-four CNS isolates identified MALDI-TOF MS cydB real-time PCR (qPCR) then qPCR presence eta, etb, sea, sec, cna, seb, sei, seq, sem, seg, see, tst toxin genes, adhesion- biofilm-associated genes (eno, ebps, fib, fnbA, coa), methicillin resistance gene (mecA). Resistance antibiotics commonly used treatment in Brazil determined using Kirby-Bauer test. Two strains positive see eta genes; mecA (1), eno (27), ebps (10), fnbA coa (5) also detected. A notable number resistant erythromycin (30), penicillin (26), cotrimoxazole (18); importantly, 10 vancomycin-resistant smaller rifampicin (8), oxacillin (7), clindamycin (5), cefepime (4), tetracycline (3), ciprofloxacin (2), chloramphenicol none gentamicin ciprofloxacin. Isolates 2 (13 isolates), 3 4 5 6 (1) Taken together, our findings suggest may not be significant cause even buffaloes, but they reservoir antibiotic genes.