The Estimation of Extracellular Space of Brain Tissue in Vitro

摘要: The measurement of extracellular space in central nervous tissue presents unresolved difficulties both methodology and interpretation data. Van Harreveld’s book (1966a) contains an excellent discussion the problem. There is still no consensus on whether there any appreciable living brain. Measurements by reputable workers range from little or none, that is, 5% at most, through a moderate amount, about 10%, to same as other soft tissues, say 15–30%, even higher. Brain slices vitro system preparations have which may be 60% more final wet weight, when measured marker methods. Because these are invariably swollen —possibly because dearth connective brain—this finding compatible with absence vivo. swelling vitro, blood-brain barrier vivo, make it impossible check vivo measurements against each other. depends extent swelling, turn preparation its treatment, details method used. can large discrepancies between results different for what appear similar preparations.