Late mitotic failure in mice lacking Sak, a polo-like kinase.

摘要: Abstract Polo-like kinases in yeast, flies, and mammals regulate key events mitosis. Such include spindle formation at G2/M, the anaphase-promoting complex (APC) exit from mitosis, cleavage structure cytokinesis, DNA damage checkpoints G2/M. are distinguished by two C-terminal polo box (pb) motifs, which localize enzymes to mitotic structures. We previously identified Sak, a novel polo-like kinase found Drosophila mammals. Here, we demonstrate that Sak has functional pb domain localizes enzyme nucleolus during G2, centrosomes furrow cytokinesis. To study role of embryo development, generated null allele, first be mutated mice. −/− embryos arrested after gastrulation E7.5, with marked increase apoptotic cells. displayed cells late anaphase or telophase continued express cyclin B1 phosphorylated histone H3. Our results suggest is required for APC-dependent destruction mitosis postgastrulation embryo.