The Molecular Structure of C4 and Its Cleavage Products

摘要: Since the cleavage products of C4 are guides to classical pathway activation, we have extended our previous studies (Fed. Proc. 34:965, 1975; Immunochemistry, in press, 1975) order characterize chain structure and amino termini produced by further degradation C4b inactivator (C4bI) combined with a high molecular weight (10S) heat diisopropyl fluorophosphate resistant factor. was purified from plasma newly developed method. is obtained yields (25 mg 800 ml plasma), stability, specific activity. The three proposed Schreiber Muller-Eberhard confirmed, NH 2 -terminus α γ chains Glu, whereas that β-chain not detected. By isoelectric focusing, found consist at least five components an point 5.5 5.8. genetic basis for this micro-heterogeneity under investigation.