Peanut agglutinin (PNA): binding and stimulation of bovine intestinal and peripheral blood leukocytes.

摘要: The use of peanut agglutinin (PNA) as a reliable marker for bovine T lymphocytes well its in vitro lymphoblastogenic capacity were investigated and compared to those concanavalin-A (ConA). binding ability fluorescein isothiocyanate conjugated PNA (FITC-PNA) FITC-ConA leukocytes isolated from peripheral blood (PBL) the intraepithelium (IEL), lamina propria (LPL) Peyer's patches (PPL) small intestinal mucosa five normal adult cows (n = 5) was analyzed using laser flow cytometry (LFC) fluorescence microscopy. Monoclonal antibodies (mAb) specific cells (B26A), B (PIg45A), "null" (B7A1) monocytes/granulocytes (DH59B) employed determine phenotype cell lineage(s) expressing surface receptor(s). There no significant variations (P greater than 0.1) proportion PNA-binding PBL (76%), PPL (77%), IEL (79%) LPL (81%) even though there differences between percentages B26A+ (26%) (38%) less 0.001) (44%) (57%) 0.01). These studies clearly indicate that other bind PNA. Although proportions enriched PP-B (30%) PP-plastic adherent significantly lower PP-T (95%), results indicated reasonable number non-T can specifically FITC-PNA. Additional support obtained by similar observed with equivalent subsets PBL. Using lymphoblastogenesis, stimulating varied various populations 0.001 PBL; P 0.02 PBL). In addition, marked on each leukocyte population 0.01 IEL). Concanavalin A which bound approximately 100% population, revealed variation mitogenic activity but not 0.1). finding some cells, monocytes/macrophages possibly granulocytes disputes reliability marker. Furthermore, abilities ConA are necessarily correlated their capacities.