Assessment of Organophosphate and Carbamate Pesticide Residues in Cigarette Tobacco with a Novel Cell Biosensor

摘要: The conventional analysis of pesticide residues in analytical commodities, such as tobacco and products is a labor intensive procedure, since it necessary to cover wide range different chemicals, using single procedure. Standard methods include extensive sample pretreatment (with solvent extraction partitioning phases) determination by GC HPLC achieve the selectivity sensitivity for classes compounds under detection. As consequence, current provide limited capacity. In present study, we report on development novel cell biosensor detecting organophosphate carbamate tobacco. sensor based neuroblastoma N2a cells measurement changes membrane potential, according working principle Bioelectric Recognition Assay (BERA). presence detected degree inhibition acetylcholine esterase (AChE). instantly responded both organophoshate chlorpyriphos carbaryl concentration-dependent pattern, being able detect one part per billion (1 ppb). Additionally, leaf samples (in blended dry form) were analyzed with methods, double-blind protocol. Pesticide caused considerable hyperpolarization immobilized sensor, indicated increase negative which was clearly distinguishable from sensor's response against pesticide-free control samples. observed quite reproducible, an average variation +5,6%. Fluorescence microscopy observations showed that treatment either chlorpyrifos or associated increased [Ca²+]cyt . offers fresh perspectives ultra-rapid, sensitive low-cost monitoring well other food agricultural commodities.