Single-cell profiling of the developing mouse brain and spinal cord with split-pool barcoding

作者: Alexander B. Rosenberg , Charles M. Roco , Richard A. Muscat , Anna Kuchina , Paul Sample

DOI: 10.1126/SCIENCE.AAM8999

关键词:

摘要: To facilitate scalable profiling of single cells, we developed split-pool ligation-based transcriptome sequencing (SPLiT-seq), a single-cell RNA-seq (scRNA-seq) method that labels the cellular origin of RNA through combinatorial barcoding. SPLiT-seq is compatible with fixed cells or nuclei, allows efficient sample multiplexing, and requires no customized equipment. We used SPLiT-seq to analyze 156,049 single-nucleus transcriptomes from postnatal day 2 and 11 mouse brains and spinal cords. More than 100 cell types were …

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