Grouper (Epinephelus coioides) IRAK-4 regulates activation of NF-κB and expression of inflammatory cytokines in grouper spleen cells.

摘要: Abstract IRAK-4 is a serine/threonine kinase that can bind to interleukin-1 receptor induced by interleukin-1. It plays key role in the Toll-like signaling pathway and involved innate adaptive immune responses. In this study, piscine significantly activated nuclear factor (NF)-κB grouper spleen cells. Grouper (Epinephelus coioides) (EcIRAK-4) co-localized with EcMyD88 did not impair EcMyD88-dependent NF-κB activation. Different doses of EcIRAK-4 caused different degrees translocation transcription p65 subunit, it multiple pro-inflammatory cytokines. Using expression vectors deletion domains or mutations at important sites EcIRAK-4, we found domain necessary for its signal transduction function. The conserved amino acid performed functions similar those mammals, grouper-specific acids such as E339 also played roles. These findings provide information about functional characteristics lower vertebrates.