MicroRNA-125b regulates osteogenic differentiation of mesenchymal stem cells by targeting Cbfβ in vitro.
作者： Ke Huang , Jingshu Fu , Wei Zhou , Wei Li , Shiwu Dong
DOI: 10.1016/J.BIOCHI.2014.02.005
关键词:
摘要: Abstract Differentiation of mesenchymal stem cells (MSCs) into a specific lineage is firmly and precisely regulated via crucial transcription factors signaling cascades, but the accurate mechanisms still need to be revealed. MicroRNAs (miRNA) negativity regulates target mRNA protein synthesis regulate various kinds biological processes. In present study we investigate miRNAs mediated regulatory osteoblastic differentiation in C3H10T1/2 identified that level miR-125b expression was obviously decreased compared with undifferentiated ones during process. Subsequently, dual-luciferase reporter gene assay data demonstrated targets putative binding site 3′-UTR Cbfβ gene, key factor for osteogenesis. We observed over interferential down-regulate over-expression decrease levels three marker genes, alkaline phosphatase (ALP), osteocalcin (OCN), osteopontin (OPN) by BMP-2-induced, whereas, anti-miR-125b increased these genes hence up-regulated Cbfβ. It concluded from result directly targeting indirectly acting on Runx2 at an early stage differentiation.
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