Detection of KIT and FLT3 Mutations in Acute Myeloid Leukemia with Different Subtypes

摘要: Background: Mutations in KIT and fms-like tyrosine kinase 3 genes lead to uncontrolled proliferation of leukemic cells with a poor prognosis. Since, data concerning the incidence associations patients characteristics vary amongst different studies, aim present study is identify quantify frequency mutations Iranian suffering from acute myeloid leukemia. Methods: Internal tandem duplication D835 gene leukemia were studied through polymerase chain reaction reaction-RFLP analysis. Amplified products for point mutation D816 have also been identified technique. The exon 8 detected by using PCR Conformational Sensitive Gel Electrophoresis techniques, amplified confirmed sequencing techniques. Results: occurred 18% 6% AML patients, respectively. Frequencies 1.4% 4.7% patients. These results substantially various subclasses French-American-British classification. Conclusion: This revealed that approximately 30% either or genetic mutations. presence was significantly associated M3 morphology M2 M4 subtypes.