Metallothionein IIA Is Up-Regulated by Hypoxia in Human A431 Squamous Carcinoma Cells

摘要: The expression of metallothionein IIA (MT-IIA) was investigated in A431 human squamous carcinoma cells exposed to hypoxia (pO < or = 0.01% atmospheric pO2) and subsequent reoxygenation. Northern analysis showed that MT-IIA mRNA levels were significantly increased during 14 h Western blotting confirmed total MT protein also response these stresses. Evidence the transcriptional control hypoxic reoxygenated found using a 0.2-kilobase sequence proximal 5'-regulatory region gene chloramphenicol acetyltransferase reporter construct. Thus promoter appears contain element(s). These observations indicate may have an important role stress responses solid tumors.