Triangulation of methods using insect cell lines to investigate insecticidal mode-of-action.

摘要: BACKGROUND This study investigated three in vitro models to assist elucidating possible mode-of-action, which could be adopted evaluate insecticidal activity of complex, unknown, or multi-constituent formulations. We used a combination absorbance spectrometry, confocal scanning laser microscopy and microelectrode ion flux estimation (MIFE) provide insight into potential target sites for insecticides. two insect cell lines evaluated pyrethroid RESULTS observed that the produced distinctly different responses. Drosophila melanogaster D.mel-S2 line was useful model monitor changes, resulting from insecticides with neural toxicity; however, it less determine some metabolic pathway indicators toxic stress. Conversely, Spodoptera frugiperda Sf9 acute reactive oxygen species (ROS) response insecticide treatments, but not highly responsive electrophysiological experiments. also showed natural, botanical extract pyrethrum elicited Na+ , Cl- Ca2+ fluxes than its synthetic, single constituent analogues, α-cypermethrin esfenvalerate. These methods spectrometry measuring growth inhibition plus mortality assays shed light on cytotoxic responses differing lines. CONCLUSION research highlights importance using multiple types interdisciplinary better mode action. is especially pertinent novel biopesticide discovery, as underlying mechanisms toxicity initial screening processes are likely unknown.