Posttranscriptional regulation of the c-myb proto-oncogene in estrogen receptor-positive breast cancer cells.
作者： Jean M. Gudas , Mikio Oka , Kenneth H. Cowan , Regina C. Klein
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摘要: We have determined that expression of the c-myb proto-oncogene is associated with estrogen receptor (ER) status and not tumor progression in human breast epithelial cells. Analysis normal, immortalized, nontumorigenic, tumorigenic mammary cells showed only ER+ cell lines expressed readily detectable levels mRNA a Mr 75,000 protein was same size as transcripts products present hematopoietic In this report we show are down-regulated during withdrawal. A 20-fold increase observed upon addition beta-estradiol to culture medium. Nuclear run-on transcription analyses transcribed at rate presence absence estrogen, suggesting accumulation regulated posttranscriptional level. To provide additional evidence dependent on ER expression, examined MCF-7 selected for resistance antineoplastic drugs. decreased concomitant loss expression. Moreover, modulated by ER-, MDA-MB-231 stably transfected gene. When considered together, these data indicate estrogens further suggest plays role biology
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