Activation of Microglia by Histamine and Substance P

摘要: Background: Activated microglia perform many of the immune effector functions typically associated with macrophages. However, regulators involved in microglial activation are not well defined. Because play a pivotal role surveillance CNS, we studied effect neuromediators histamine and substance P on microglia. Methods: The induction by was examined using primary cultured Fluorescent images were acquired confocal microscope. levels TNF-α IL-6 measured commercial ELISA kit. Intracellular reactive oxygen species (ROS) determined dichlorodihydrofluorescein oxidation. mitochondrial membrane potential assessed MitoProbe™ JC-1 assay Results: We found that able to stimulate subsequent production ROS proinflammatory factors IL-6. These effects partially abolished antagonists receptors H1 H4 NK-1, NK-2 NK-3. Histamine induced depolarization Conclusions: results indicate SP can trigger release pro-inflammatory from microglia, thus contributing development microglia-mediated inflammation brain.