Electron microscope heteroduplex mapping of naphthalene oxidation genes on the NAH7 and SAL1 plasmids.
作者： K.-M. Yen , M. Sullivan , I.C. Gunsalus
DOI: 10.1016/0147-619X(83)90013-6
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摘要: Abstract Introduction of the transposon Tn5 to serve as a marker allows electron microscope heteroduplex mapping naphthalene oxidation genes on ~83-kb NAH7 and related ~85-kb SAL1 plasmids. The microscope-mapped gene positions plasmid are in close agreement with those mapped previously by restriction digestion. can be considered mutant which fails direct conversion salicylate because mutational block but retains intact coding sequences for oxidation. Analysis molecules formed between NAH7::Tn5 EcoRI fragments known NAH7/SAL1 homology strongly suggest that DNA is completely homologous except ~2.5-kb segment constituting most nahA replaced ~4.6-kb nonhomologous DNA.
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sci-hub.se 参考文章(26)
James B. Johnston, I.C. Gunsalus, Isolation of metabolic plasmid DNA from Pseudomonas putida. Biochemical and Biophysical Research Communications. ,vol. 75, pp. 13- 19 ,(1977) , 10.1016/0006-291X(77)91282-7
M Hermann, G K Garg, I C Gunsalus, Fertility factors in Pseudomonas putida: selection and properties of high-frequency transfer and chromosome donors. Journal of Bacteriology. ,vol. 137, pp. 28- 34 ,(1979) , 10.1128/JB.137.1.28-34.1979
A. M. Chakrabarty, Genetic Basis of the Biodegradation of Salicylate in Pseudomonas Journal of Bacteriology. ,vol. 112, pp. 815- 823 ,(1972) , 10.1128/JB.112.2.815-823.1972
T Nakazawa, E Hayashi, T Yokota, Y Ebina, A Nakazawa, Isolation of TOL and RP4 recombinants by integrative suppression. Journal of Bacteriology. ,vol. 134, pp. 270- 277 ,(1978) , 10.1128/JB.134.1.270-277.1978
C J Duggleby, S A Bayley, M J Worsey, P A Williams, P Broda, Molecular sizes and relationships of TOL plasmids in Pseudomonas. Journal of Bacteriology. ,vol. 130, pp. 1274- 1280 ,(1977) , 10.1128/JB.130.3.1274-1280.1977
D. E. Berg, J. Davies, B. Allet, J. D. Rochaix, Transposition of R factor genes to bacteriophage lambda Proceedings of the National Academy of Sciences of the United States of America. ,vol. 72, pp. 3628- 3632 ,(1975) , 10.1073/PNAS.72.9.3628
K. M. Yen, I. C. Gunsalus, Plasmid gene organization: naphthalene/salicylate oxidation. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 79, pp. 874- 878 ,(1982) , 10.1073/PNAS.79.3.874
A. M. Chakrabarty, D. A. Friello, L. H. Bopp, Transposition of plasmid DNA segments specifying hydrocarbon degradation and their expression in various microorganisms Proceedings of the National Academy of Sciences of the United States of America. ,vol. 75, pp. 3109- 3112 ,(1978) , 10.1073/PNAS.75.7.3109
W. H. Bradshaw, H. E. Conrad, E. J. Corey, I. C. Gunsalus, D. Lednicer, MICROBIOLOGICAL DEGRADATION OF (+)-CAMPHOR Journal of the American Chemical Society. ,vol. 81, pp. 5507- 5507 ,(1959) , 10.1021/JA01529A060