Isolation of metabolic plasmid DNA from Pseudomonas putida.
作者： James B. Johnston , I.C. Gunsalus
DOI: 10.1016/0006-291X(77)91282-7
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摘要: Abstract Metabolic plasmids conferring on Pseudomonas putida the aromatic growth phenotypes naphthalene, Nah+, salicylate, Sal+, or toluate, Tol+, have been isolated as covalently closed circular DNA in 100 μg amounts. Plasmid was banded CsCl-ethidium bromide density gradients and sedimentation rates measured sucrose by analytical centrifuge. The plasmid sizes found, millions, were /NAH 42, /SAL 43, /TOL 55, 42. Transformation of metabolic free P. with confirmed respective pathway gene contents.
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