The effect of actin disrupting agents on contact guidance of human embryonic stem cells
作者： Sharon Gerecht , Christopher J. Bettinger , Zhitong Zhang , Jeffrey T. Borenstein , Gordana Vunjak-Novakovic
DOI: 10.1016/J.BIOMATERIALS.2007.05.027
关键词:
摘要: Mammalian cells respond to their substrates by complex changes in gene expression profiles, morphology, proliferation and migration. We report that substrate nanotopography alters morpohology of human embryonic stem (hESCs). Fibronectin-coated poly(di-methyl siloxane) with line-grating (600nm ridges 600nm spacing 600+/-150nm feature height) induced hESC alignment elongation, mediated the organization cytoskeletal components including actin, vimentin, alpha-tubulin, reduced proliferation. Spatial polarization gamma-tubulin complexes was also observed response nanotopography. Furthermore, addition actin disrupting agents attenuated proliferative effects These findings further demonstrate importance interplay between cytoskeleton interactions as a key modulator morphological cellular hESCs on
elsevier.com
sci-hub.se 参考文章(36)
Benjamin Geiger, Alexander Bershadsky, Roumen Pankov, Kenneth M. Yamada, Transmembrane crosstalk between the extracellular matrix and the cytoskeleton Nature Reviews Molecular Cell Biology. ,vol. 2, pp. 793- 805 ,(2001) , 10.1038/35099066
Joseph Itskovitz-Eldor, Maya Schuldiner, Dorit Karsenti, Amir Eden, Ofra Yanuka, Michal Amit, Hermona Soreq, Nissim Benvenisty, Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers. Molecular Medicine. ,vol. 6, pp. 88- 95 ,(2000) , 10.1007/BF03401776
Russell J Composto, George K Toworfe, Paul Ducheyne, Irving M Shapiro, Christopher S Adams, Effect of surface activated poly(dimethylsiloxane) on fibronectin adsorption and cell function Materials Research Society Symposium Proceedings. pp. 131- 135 ,(2004)
Donald M. Brunette, Laisheng Chou, James D. Firth, Veli-Jukka Uitto, Substratum surface topography alters cell shape and regulates fibronectin mRNA level, mRNA stability, secretion and assembly in human fibroblasts Journal of Cell Science. ,vol. 108, pp. 1563- 1573 ,(1995) , 10.1242/JCS.108.4.1563
Ana I Teixeira, George A Abrams, Paul J Bertics, Christopher J Murphy, Paul F Nealey, Epithelial contact guidance on well-defined micro- and nanostructured substrates Journal of Cell Science. ,vol. 116, pp. 1881- 1892 ,(2003) , 10.1242/JCS.00383
R.G. Flemming, C.J. Murphy, G.A. Abrams, S.L. Goodman, P.F. Nealey, Effects of synthetic micro- and nano-structured surfaces on cell behavior Biomaterials. ,vol. 20, pp. 573- 588 ,(1999) , 10.1016/S0142-9612(98)00209-9
Adam Curtis, Chris Wilkinson, Topographical control of cells Biomaterials. ,vol. 18, pp. 1573- 1583 ,(1997) , 10.1016/S0142-9612(97)00144-0
Elmar Schiebel, γ-tubulin complexes: binding to the centrosome, regulation and microtubule nucleation Current Opinion in Cell Biology. ,vol. 12, pp. 113- 118 ,(2000) , 10.1016/S0955-0674(99)00064-2
Gavin Thurston, Bruno Jaggi, Branko Palcic, Measurement of cell motility and morphology with an automated microscope system Cytometry. ,vol. 9, pp. 411- 417 ,(1988) , 10.1002/CYTO.990090502
X.F. Walboomers, H.J.E. Croes, L.A. Ginsel, J.A. Jansen, Contact guidance of rat fibroblasts on various implant materials. Journal of Biomedical Materials Research. ,vol. 47, pp. 204- 212 ,(1999) , 10.1002/(SICI)1097-4636(199911)47:2<204::AID-JBM10>3.0.CO;2-H