作者: B. D. Stollar , Chichi Huang
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摘要: Certain Ig VH gene segments, with few or no mutations, recur frequently in natural autoantibodies, fetal antibodies, and products of B cell tumors. The goal this study was to determine whether similar segment usage occurs normal human adult PBL. Extending previous analyses, 105 randomly picked H chain V region clones representative cDNA libraries from PBL were sequenced. Clones from: IgM IgG one RNA sample a adult; second library the same subject 11 mo later; subject. Although some had clear evidence mutation, 48 77 (62%) shared 99% more identity known germline most these mutations CDR3 portion JH segment. expressed autoantibodies occurred at high frequency libraries. Fourteen segments identical portions DH sequences; two such N nucleotides VHDH DHJH junctions. IgG-encoding sequences than IgM-encoding sequences. not random. circulating population may represent distinct compartment, large proportion cells those autoantibody repertoire. Polyreactive serve screening function, binding delivering diverse Ag secondary lymphoid tissues where highly selective antibodies are formed foreign self-Ag.