Gypenosides Ameliorate Carbon Tetrachloride-Induced Liver Fibrosis by Inhibiting the Differentiation of Hepatic Progenitor Cells into Myofibroblasts

摘要: Gypenosides (GPs), the predominant components of Gynostemma pentaphyllum, exert antifibrotic effects; however, mechanisms underlying their ability to ameliorate liver fibrosis are unclear. Liver was induced in C57BL/6 mice via subcutaneous injection 10% carbon tetrachloride (CCl[Formula: see text] three times a week for two weeks. Then, CCl4 administered conjunction with intragastric GPs another For vitro analyses, WB-F344, hepatatic progenitor cells (HPCs) were treated transforming growth factor beta 1 (TGF-[Formula: text]1) or without 48[Formula: text]h. The results showed that alanine aminotransferase (ALT) and aspartate transaminase (AST) activity, deposition collagen, hydroxyproline content, expression alpha-smooth muscle actin ([Formula: text]-SMA) collagen type I (Col I) significantly decreased after treatment text], [Formula: text]). In 5M group, HPC markers, Sox9 cytokeratin 19 (CK19), increased compared normal GPs-treated group Immunostaining number text]-SMA double-positive higher than but addition caused this cell decrease. WB-F344 cells, Col TGF-[Formula: whereas markedly levels text]R1 reduced both vivo vitro. conclusion, ameliorated CCl4-induced inhibition signaling, consequently inhibiting differentiation HPCs into myofibroblasts.