A correlation between soluble brain nitric oxide synthase and NADPH-diaphorase activity is only seen after exposure of the tissue to fixative.
作者： Takahiro Matsumoto , Masaki Nakane , Jennifer S. Pollock , Jane E. Kuk , Ulrich Förstermann
DOI: 10.1016/0304-3940(93)90673-9
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摘要: Abstract In histochemical studies using fixed brain tissue, NADPH-diaphorase has been found to be colocalized with soluble nitric oxide synthase. the present study, fresh tissues from eight different regions of rat brain, activity was mostly in particulate fraction, whereas most synthase located cytosolic fraction. Also, distribution among that Pretreatment fractions paraformaldehyde virtually abolished 40–60% remained intact These results suggest during fixation is inactivated and only some associated remains intact.
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