Rat brain NADPH-dependent diaphorase. A possible relationship to cytochrome P450 reductase.
作者： M.C. Kemp , D.R. Kuonen , A. Sutton , P.J. Roberts
DOI: 10.1016/0006-2952(88)90302-4
关键词:
摘要: Abstract An enzyme (NADPH-dependent diaphorase) present in rat brain microsomes has been solubilised and shown to utilise both nitrobluetetrazolium cytochrome c as electron acceptors, when reduced by NADPH. The kinetics of the have determined using ( K m = 1.3 μ M), NADPH 1.4 M) V max (4.7 nmol/min/mg microsome protein). subunit M r is approximately 73,000 D that native 170,000–180,000 D, indicating probably a dimer. Evidence also provided show flavoprotein, it equimolar amounts FAD FMN with respect concentration. It seems possibility diaphorase may be P 450 reductase, EC 1.6.2.4.
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